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Impress
Your Friends, Win Adulations |
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Converting from micrograms (µg) to nanomoles (nmol) to picomoles (pmol) to nanomolar to milimolar can be confusing. The goal of this short tutorial is to show you a way to easily inter-convert these values while standing in front of your mass spectrometer talking to a client. |
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Remember: M > mM > µM > nM > pM > fM |
| 1.) Quantities: An easy method to convert nmols to µgs |
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Easy trick number one. |
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1 nmol of a compound is equal to the kDa mass of
the compound converted to µgs. For example 1 nmol of a 50 kDA protein is 50 µg. |
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| The Proof: |
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1 mole of a 50 kDa protein is |
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50,000 g |
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1 millimole is |
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50 g or 50,000,000 µg |
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1 micromole is |
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50,000 µg |
| 1 nanomole is |
| 50 µg |
| 2.) Concentrations: An easy method to convert molar concentration to absolute quantities. (because as LC/MS people we always want to know the absolute amount going on to the HPLC column.) |
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| Easy trick number two. |
| A 1 millimolar solution of a compound is equal to the kDa mass of
the compound converted to µgs/µl. For example a 1 millimolar solution of a 50 kDa protein is 50 µg/µl. |
| The Proof: |
A 1 molar solution of a 50 kDa protein is |
50,000 g/L |
A 1 millimolar solution of a 50 kDa protein is |
50 g/L
or 50 ug/ul |
| Q: For example
if someone says, "I have 10 µLs of a 10 mM solution of a 50 kDa
protein", how many µgs does he have?
A: A one mM solution would be 50 µg/µl therefore a 10 mM solution is 10 X 50 µg/µl = 500 µg/µl and then 10 µl of such a solution is 10 X 500 µg/µl = 5000 µg |
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In Summary
1.) 1 nmol of a 50 kDa compound is 50 µg |
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