ENTRY KBBOA2 #type complete iProClass View TITLE beta-casein precursor - bovine ORGANISM #formal_name Bos primigenius taurus #common_name cattle DATE 24-Apr-1984 #sequence_revision 12-May-1995 #text_change 09-Jul-2004 ACCESSIONS I45873; B29087; S01860; A25846; S02429; A90489; A91191; B91192; C91192; D91192; A90739; I46963; A91413; A03110 REFERENCE I45873 #authors Bonsing, J.; Ring, J.M.; Stewart, A.F.; Mackinlay, A.G. #journal Aust. J. Biol. Sci. (1988) 41:527-537 #title Complete nucleotide sequence of the bovine beta-casein gene. #cross-references MUID:90147279; PMID:3271384 #accession I45873 ##status translated from GB/EMBL/DDBJ ##molecule_type DNA ##residues 1-81,'H',83-224 ##label BON ##cross-references UNIPROT:P02666; UNIPARC:UPI000016C2D0; GB:M55158; NID:g162804; PIDN:AAA30431.1; PID:g162805 REFERENCE A93062 #authors Stewart, A.F.; Bonsing, J.; Beattie, C.W.; Shah, F.; Willis, I.M.; Mackinlay, A.G. #journal Mol. Biol. Evol. (1987) 4:231-241 #title Complete nucleotide sequences of bovine alpha-s2- and beta-casein cDNAs: comparisons with related sequences in other species. #cross-references MUID:88188989; PMID:2833669 #accession B29087 ##status translation not shown ##molecule_type mRNA ##residues 1-224 ##label STE ##cross-references UNIPARC:UPI0000126FD7; GB:M16645; NID:g162930; PIDN:AAA30480.1; PID:g162931 ##experimental_source A2 variant REFERENCE S01860 #authors Baev, A.A.; Smirnov, I.K.; Gorodetskii, S.I. #journal Mol. Biol. (1987) 21:214-222 #title Primary structure of bovine beta-casein cDNA. #accession S01860 ##molecule_type mRNA ##residues 1-81,'H',83-224 ##label BAE ##cross-references UNIPARC:UPI000016C2D0; EMBL:X06359; NID:g171; PIDN:CAA29658.1; PID:g757752 ##experimental_source A1 variant ##note this paper is a translation of the Russian paper published in Mol. Biol. Moscow (1987) 21: 255-265 REFERENCE A25846 #authors Jimenez-Flores, R.; Kang, Y.C.; Richardson, T. #journal Biochem. Biophys. Res. Commun. (1987) 142:617-621 #title Cloning and sequence analysis of bovine beta-casein cDNA. #cross-references MUID:87128158; PMID:3814153 #accession A25846 ##molecule_type mRNA ##residues 1-107,'L',109-151,'PL',154-209,'Q',211-224 ##label JIM ##cross-references UNIPARC:UPI000016C2CF; GB:M15132; NID:g162796; PIDN:AAA30430.1; PID:g162797 REFERENCE S02429 #authors Carles, C.; Huet, J.C.; Ribadeau-Dumas, B. #journal FEBS Lett. (1988) 229:265-272 #title A new strategy for primary structure determination of proteins: application to bovine beta-casein. #cross-references MUID:88152252; PMID:3278933 #accession S02429 ##molecule_type protein ##residues 16-81,'H',83-224 ##label CAR ##cross-references UNIPARC:UPI0000174050 ##experimental_source A1 variant REFERENCE A90489 #authors Yan, S.B.; Wold, F. #journal Biochemistry (1984) 23:3759-3765 #title Neoglycoproteins: in vitro introduction of glycosyl units at glutaminesin beta-casein using transglutaminase. #cross-references MUID:85000478; PMID:6148101 #accession A90489 ##molecule_type protein ##residues 16-224 ##label YAN ##cross-references UNIPARC:UPI0000174051 REFERENCE A91191 #authors Ribadeau-Dumas, B.; Brignon, G.; Grosclaude, F.; Mercier, J.C. #journal Eur. J. Biochem. (1972) 25:505-514 #title Structure primaire de la caseine beta bovine. #cross-references MUID:72233212; PMID:4557764 #accession A91191 ##molecule_type protein ##residues 16-131,'Q',133-151,'PL',154-189,'E',191-209,'Q',211-224 ##label RIB ##cross-references UNIPARC:UPI0000174052 ##experimental_source A2 variant ##note article in French with an English abstract REFERENCE A91192 #authors Grosclaude, F.; Mahe, M.F.; Mercier, J.C.; Ribadeau-Dumas, B. #journal Eur. J. Biochem. (1972) 26:328-337 #title Caracterisation des variants genetiques des caseines alpha-S1 et beta bovines. #cross-references MUID:72214259; PMID:5064450 #note article in French with an English abstract #accession B91192 ##molecule_type protein ##residues 16-81,'H',83-131,'Q',133-151,'PL',154-189,'E',191-209,'Q', 211-224 ##label VA1 ##cross-references UNIPARC:UPI0000174053 ##experimental_source A1 variant #accession C91192 ##molecule_type protein ##residues 16-81,'H',83-131,'Q',133-136,'R',138-151,'PL',154-189,'E', 191-209,'Q',211-224 ##label VAB ##cross-references UNIPARC:UPI0000174054 ##experimental_source B variant #accession D91192 ##molecule_type protein ##residues 16-51,'K',53-81,'H',83-131,'Q',133-151,'PL',154-189,'E', 191-209,'Q',211-224 ##label VAC ##cross-references UNIPARC:UPI0000174055 ##experimental_source C variant ##note this variant lacks a phosphate group on 50-Ser REFERENCE A90739 #authors Ribadeau-Dumas, B.; Grosclaude, F.; Mercier, J.C. #journal C. R. Acad. Sci. Hebd. Seances Acad. Sci. D (1970) 270:2369-2372 #title Localisation dans la chaine peptidique de la caseine beta bovine de la substitution His/Gln differenciant les variants genetiques A2 et A3. #cross-references MUID:71252171; PMID:4997616 #note article in French with an English abstract #accession A90739 ##molecule_type protein ##residues 118-120,'Q',122-124 ##label VA3 ##cross-references UNIPARC:UPI0000174056 ##experimental_source A3 variant REFERENCE I46963 #authors Simons, G.; van den Heuvel, W.; Reynen, T.; Frijters, A.; Rutten, G.; Slangen, C.J.; Groenen, M.; de Vos, W.M.; Siezen, R.J. #journal Protein Eng. (1993) 6:763-770 #title Overproduction of bovine beta-casein in Escherichia coli and engineering of its main chymosin cleavage site. #cross-references MUID:94068382; PMID:8248100 #accession I46963 ##status translated from GB/EMBL/DDBJ ##molecule_type mRNA ##residues 1-120,'Q',122-224 ##label SIM ##cross-references UNIPARC:UPI000016C627; GB:S67277; NID:g459291; PIDN:AAB29137.1; PID:g459292 ##experimental_source A3 variant REFERENCE A91413 #authors Grosclaude, F.; Mahe, M.F.; Voglino, G.F. #journal FEBS Lett. (1974) 45:3-5 #title Le variant beta-E et le code de phosphorylation des caseines bovines. #cross-references MUID:75005247; PMID:4411121 #note article in French with an English abstract #accession A91413 ##molecule_type protein ##residues 48-50,'K',52-63 ##label VAE ##cross-references UNIPARC:UPI0000174057 ##experimental_source E variant ##note 50-Ser is phosphorylated COMMENT The sequence shown is the A2 variant. GENETICS #introns 17/3; 26/3; 35/3; 43/3; 57/3; 223/3 CLASSIFICATION #superfamily beta-casein KEYWORDS milk; phosphoprotein FEATURE 1-15 #domain signal sequence #status predicted #label SIG\ 16-224 #product beta-casein #status experimental #label MAT\ 30,32,33,34 #binding_site phosphate (Ser) (covalent) (by casein kinase II) #status experimental\ 50 #binding_site phosphate (Ser) (covalent) (by casein kinase II) (partial) #status experimental SUMMARY #length 224 #molecular-weight 25107 #checksum 8112 SEQUENCE 5 10 15 20 25 30 1 M K V L I L A C L V A L A L A R E L E E L N V P G E I V E S 31 L S S S E E S I T R I N K K I E K F Q S E E Q Q Q T E D E L 61 Q D K I H P F A Q T Q S L V Y P F P G P I P N S L P Q N I P 91 P L T Q T P V V V P P F L Q P E V M G V S K V K E A M A P K 121 H K E M P F P K Y P V E P F T E S Q S L T L T D V E N L H L 151 P L P L L Q S W M H Q P H Q P L P P T V M F P P Q S V L S L 181 S Q S K V L P V P Q K A V P Y P Q R D M P I Q A F L L Y Q E 211 P V L G P V R G P F P I I V