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Table of Contents
Surfing Hints: Phenyx A software platform for the identification and characterization of proteins and peptides from mass spectrometry data. Developed by GeneBio in collaboration with the Swiss Institute of Bioinformatics (SIB). Proteomic Tools from the Institute for Systems Biology: peptide fragment calculator, protein digester, DNA translator, composition calculator. ChemCalc - a chemical calculator written entirely in Java by Michal Krompiec. Calculates isotopic distribution, elemental composition, molecular mass, exact mass and molecular formula. Buffers for pH control. Use a java calculator to design a buffer with specified pH, concentration, and ionic strength. SearchXLinks SearchXLinks, a program for analyzing mass spectrometric experiments carried out on modified, cross-linked and digested proteins. This includes identification of peptides linked via disulfide bridges. They provide a fully functional free web based version and they also sell a standalone version. MS-Bridge linked peptide search of MS data at the UCSF Protein Prospector web site. Useful in identifying disulfide lined peptides.
Mass Spectrometry Consultants and Contract Labs Bay Bioanalytical Laboratory, Inc. They do electrospray, APCI, HPLC, GC, CE, Spectroscopy, Method Validation. Visit their services page for more detail. (GLP/cGMP compliant) M-Scan Mass Spectrometry Consultants and Analysts. "Areas of specialist expertise include Biotechnology and Environmental/Petroleum Analysis. In addition to mass spectrometry, other analytical services such as GC, HPLC, gas phase sequencing, amino acid analysis, carbohydrate composition and linkage analysis are routinely available." Matt
Sweeney Mass Spec Consulting
Training/Operations/Consulting/Method Development LC/MS Pharmacokinetics,
Peptides, Proteins, Metabolism, Maintenance Classes, Specialist in Finnigan
Equipment and Software. IonSource is proud to host Matt's Perl for
Proteomics Page on our web site. Novatia Corporation Novatia is a mass spectrometry consulting company with Mark Hail as the chief consultant The Novatia web site provides an open forum to help people get answers in the areas of LC/MS, bio-MS, and high-throughput pharmaceutical applications of MS. The web site contains hot topics like; which mass spectrometer system is best for proteomics and tips for using SEQUEST. Pheonix International Life Sciences, Inc. now MDS Pharma Services, They do it all from lead drug candidate ID through all phases of clinical trials. (GLP/cGMP compliant) Stanford University Mass Spectrometry Core Facility This lab provides rapid protein identification from 1D and 2D SDS PAGE gels. This lab also provides quality detailed peptide mapping in conjunction with post-translational modification identification. There is also the availability of experienced mass spectrometry and proteomics consultation. This is a quality contract laboratory. Taylor Technology, Inc. "Is a privately held contract analytical laboratory focused on quantitative bioanalytical mass spectrometry services for the pharmaceutical industry. Their services are utilized across the spectrum of the drug development process from early discovery to phases III and IV." (GLP/cGMP compliant) Baktron Search a bacterial database to identify bacteria by mass. Biology Links Page. Harvard University Dept of Molecular and Cellular Biology. Many good database links. Proteome Analysis @ EBI Fine collection of protein databases; human, yeast, drosophila and many more. Entrez-PubMed National Center for Biotechnology Information. Literature databases. National Library of Medicine. ExPASy Molecular Biology Server with links to SWISS-PROT and TrEMBL "Annotated protein sequence database" and FTP: SWISS-PROT + TrEMBL download a non-redundant database and PeptIdent "PeptIdent is a tool that allows the identification of proteins using pI, Mw and peptide mass fingerprinting data." There is so much more, here is a list of links to ExPASy. National Center for Biotechnology Information, "NCBI creates public databases." Oxford Glycobiology Institute Many good proteomics and valuable database links. Protein Data Bank, PDB, "The single international repository for the processing and distribution of 3-D macromolecular structure data primarily determined experimentally by X-ray crystallography and NMR." SEQUEST MS/MS database searching tool by Jimmy Eng/John Yates. This link originates at The Scripps Research Institute
Human,
Yeast, and nonredundant Protein and DNA databases. Link to
Sequest resource. ChemFinder
Free search by chemical name, CAS number, molecular formula, or
molecular weight.
Definitions (MS),
Dictionaries and Reference Materials
General Glossary at The Electrophoresis Society Genetics Glossary The University of Edinburgh, School of Biology The Dictionary of Cell and Molecular Biology at The University of Edinburgh. HPLC Glossary from Upchurch Scientific Biochemical Society Interest Groups, Glossary of terms Glossary
of terms used in theoretical organic chemistry at the IUPAC web site. IUPAC Commission on
Atomic
Weights and Isotopic Abundances 1997
no change has been made to the 1995 atomic weights except for a footnote for
lithium. Isotopic reports on non-terrestrial data added. Invitrogen. The new seller of Novex line of electrophoresis products. Employment Listings
A History of Mass Spectrometry: The Scripps Research Institute in La Jolla, CA Description: Mass spectrometry historical retrospective. Who
Won the Nobel Prize for the Invention of the Ion Trap? You should know, try
looking here. History of Chemistry Presented by Woodrow Wilson Summer Institute. Excellent monographs on chemists from the past. History of
Electrochemistry History of Xenobiotic Metabolism Classic Papers from the History of Chemistry Classic papers posted at Diamond Bar High School Mass Spectrometry Interest Groups American Society for Mass Spectrometry (ASMS) Topics: Analytical Laboratory Managers | Biological Macromolecules | Computer Applications | Desorption Ionization | Elemental & Isotopic | Environmental Applications | Flavor, Fragrance & Foodstuffs | Fourier Transform MS | Fundamentals | History of Mass Spectrometry | Hydrocarbon & Chemical Processes | Ion Trap Mass Spectrometry | LC/MS & Related Topics | Metal Ion Coordination Chemistry | Pharmaceuticals | Polymeric Materials | Quantitation | Surface Science | Time-of-Flight MS | Young Mass Spectrometrists The Association of Biomolecular Resource Facilities Electronic Discussion Group (ABRF) British Mass Spectrometry Society Special Interest Groups (BMSS) Topics: Combinatorial Chemistry | Environmental Food and Nutrition | Fundamentals | Interface 2000 | LC/MS | Miniaturization | MS/MS | Non-covalent interactions | Trapped Ions Google Mass Spectrometry Interest Group The Mass Spectrometry Interest Group of the National Cancer Institute at Frederick, MD NIH Mass Spectrometry Discussion Group List of Regional Discussion Groups at the NCI at Frederick Web Site
Journal
of The American Society for Mass Spectrometry JASMS is accessible
on-line for members of ASMS and Chem-Web. Electrophoresis, Publisher Wiley-VCH Rapid Communications in Mass Spectrometry , Publisher Wiley InterScience. The Company of Biologists Limited Is a non-profit organization. They have three journals; Development, Journal of Cell Science, Journal of Experimental Biology. Abstracts are available for all articles and entire pdf versions for older articles. Research Group of Dwight E. Matthews, Ph.D. at the University of Vermont (UVM) Their motto: "We Do Stable Isotopes Better" Dwight is Director of the Mass Spectrometry Facility in the General Clinical Research Center, University of Vermont College of Medicine, Burlington, Vermont. Visit the proteomics page at IonSource BiotechFind More than just a search engine, a resource for the biotechnology industry. Kart00 A search engine that generates internet maps. Try searching Mass Spectrometry.
Societies The British Mass Spectrometry Society Canadian Society for Mass Spectrometry CSMS, Yearly Tandem Mass Spectrometry Workshop in Lake Louise, Alberta at the The Fairmont Chateau Lake Louise (December 1 - 4, 2004). The Electrophoresis Society "Promoting scientific advancement in electrophoretic theory and applications." Joint 19th Annual Meeting of The Electrophoresis Society (AES) and American Institute of Chemical Engineers (AIChE). Indianapolis Convention Center Indianapolis, IN Nov.3-8, 2002 The 5th Harsh-Environment Mass Spectrometry Workshop and The 2nd Miniature Vacuum Pump Workshop Presented by the JPL, Pasadena, CA, Fall, 2005 The International Mass Spectrometry Society IMSS, The next IMSC in 2003 in Edinburgh. The Italian Home page Contains links to mass spectrometry meetings in Italy and the world. Nederlandse Vereniging voor Massaspectrometrie NVMS Dutch Society for Mass Spectrometry List of Mass Spectrometry Societies Links made available through anzsms The San Francisco Bay Area Mass Spectrometry (BAMS) discussion group Swiss Group for Mass Spectrometry
Atomic
Weights and Isotopic Compositions, NIST,
Physics Laboratory Periodic Table of the Elements Faculty of Chemical Technology, Split, Croatia A Science Primer from NCBI, includes primers on: bioinformatics, genome mapping, molecular modeling, SNPs. ESTs, microarray technology, molecular genetics, phamacogenomics, phylogenetics. The University of New Mexico. Many good mass spectrometry mini tutorials: Nominal mass determinations, Exact mass (150-1000 Da), High mass accuracy of peptides, ESI of oligonucleotides, ESI of proteins, Multiply charged ions, ESI data on the QTof. Buffers for pH control. Use a java calculator to design a buffer with specified pH, concentration, and ionic strength. Carboxyl-Terminal
Sequencing Methods, by John E. Shively at the ABRF web site. Basic Mass Spectroscopy By Paul R. Young, University of Illinois at Chicago, 1996. Mass Spectrometry, Vanderbilt University, Mass Spectrometry Research Center, by Richard Caprioli and Marc Sutter, revised by Markus Stoeckli. Detailed tutorial in slide form. Theory of mass spectrometry One of the best tutorials, recommended. Presented at the University of Cambridge, Department of Chemistry by Paul Gates. Topics Covered- Ionization: EI, FAB & LSIMS, ESI, MALDI, Mass Analysis: Sector, TOF Quadrupole, FT-ICR, Ion Trap. "Introduzione alla Apettrometria di Massa" An beautiful introduction to mass spectrometry in Italian, by Prof. Carlo Mauizio Camaggi. Mass Spectrometry On-line Tutorial Under construction and provided by Kamel Harrata at Iowa State University. "Instrument and Ionization Methods Tutorial" Washington University Center for Biomedical and Bioorganic Mass Spectrometry: A NIH-Supported Resource Center. Mass Spectrometry Accuracy and Resolution from Matrix Science Ltd An Introduction to Fourier Transform Mass Spectrometry IonSpec Corporation Introduction to Enzymes, at Worthington Biochemical Corporation. HPLC Introduction at Upchurch Scientific. Visit the Chem Team for many great chemistry tutorials and chem. history. What is Microdialysis? and how can it be used in pharmacokinetics?
Stanford University Mass Spectrometry Core Facility This lab provides rapid protein identification from 1D and 2D SDS PAGE gels. This lab also provides quality detailed peptide mapping in conjunction with post-translational modification identification. There is also the availability of experienced mass spectrometry and proteomics consultation. This is a quality contract laboratory. PAF : Protein Analysis Facility at The University of Lausanne. They provide protein MS ID services, as well as education, collaborations and plan to provide new technology which will benefit the scientific community. The University of New Mexico, Mass Spectrometry in the Department of Chemistry. Many good mass spectrometry mini tutorials: Nominal mass determinations, Exact mass (150-1000 Da), High mass accuracy of peptides, ESI of oligonucleotides, ESI of proteins, Multiply charged ions, ESI data on the QTof. University Utrecht, Department of Biomolecular Mass Spectrometry The group in Utrecht applies mass spectrometry primarily to proteomics and studies into biomolecular interactions, such as protein-protein interactions. Here is a short list of the research projects that they are currently involved with: Assembly and function of flavoproteins, B-cell activation, New drug-targets in for instance asthma and cancer, Enzyme-inhibitor studies on for instance Glyoxalase I and tyrosine kinases, Protein-membrane interactions and Antibiotics function (vancomycin, nisin and colicin).
Dwight E.
Matthews Research
Group, Ph.D. at
the University of Vermont (UVM) Their motto: "We Do Stable Isotopes
Better" Dwight is Director of the Mass Spectrometry Facility in the
General Clinical Research Center, University of Vermont College of Medicine,
Burlington, Vermont.
Michael
MacCoss' Home Page at the University of
Washington
Oak Ridge
National Laboratory "ORNL is a multiprogram science and technology
laboratory managed for the U.S.
Department of Energy by UT-Battelle,
LLC. Scientists and engineers at ORNL conduct basic and applied research and
development to create scientific knowledge and technological solutions that
strengthen the nation's leadership in key areas of science."
Agilent They sell the 1100™
standard and capillary HPLC's. Bodman 800-241-8774, They sell chromatography systems and chromatography supplies. Cohesive
Technologies Specializing in systems for chromatographic solutions for
pharmacokinetic analysis. Michrom BioResources, Inc. (530) 888-6498, Michrom sells the MAGIC 2002™ HPLC plus columns traps and other HPLC and LC/MS supplies and accessories. The company founder and president is Kerry Nugent a well respected and knowledgeable chromatographer.. Mico-Tech
Scientific Inc. 408-730-8324, Located in Sunnyvale, California. They sell capillary HPLCs along with a large selection of capillary HPLC
columns and supplies. Column selection page. Rheodyne L.P. 707-588-2016, Best known as an HPLC injection valve manufacturer. Lots of information here on trouble shooting HPLC injection valves.
ThermoFinnigan They sell the
Surveyor™ HPLC, and assorted UV detectors as well as autosamplers. Upchurch Scientific 800- 426-0191, They sell chromatography products and specialize in HPLC fittings and connections. They have helpful resources like "The HPLC Center" Valco Instruments Co. Inc. Contact Info. Maybe best known as an HPLC injection valve manufacturer. The products page. Fantastic valve applications with Java enabled valves to show you all the positions, great applications and ideas. Support Page. Theys sell Injectors, detectors, valves, fittings, syringes and tubing. Vydac 800-247-0924, They sell HPLC columns ranging from capillary to preparative. My favorite peptide column is Vydac C8-300A-5 micron. (recheck this link) Vendors (MS) Bruker Daltonics They sell ion traps, MALDI TOF, FTMS and more http://www.bruker-daltonik.de/ (recheck this link) Micromass They sell single and triple quadrupole mass spectrometers, MALDI TOF, ICP mass spectrometers and more of course. New Objective They sell nano-ESI/MS accessories and more. Proxeon They sell nano-spray attachments, nano-spray needles and other tools for proteomic investigations including software. Thermo Finnigan Corporation. They sell ion traps, triple quadrupole mass spectrometers, GC/MS, ICP-MS, FTMS, magnetic sector instruments and more.
Links to More Links and Resources Gene's Now (DB Link) Graduate School of Frontier Sciences, The University of Tokyo
Links to mobile
and miniature mass spectrometer BioPortfolio.Com Sort of like a Bio Search engine.
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