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- De Novo Peptide Sequencing Tutorial
Example Spectrum part two, mid mass |
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| As we move into the mid spectrum
range the intensity of the b ions increase, see Figure 1 below. The b
ions in Figure 1 are losing water and ammonia which is consistent with S
(-18) and Q (-17) residues. Peaks consistent with a ions are
also observed at -28u from two of the b ions.
Figure 1. |
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The predominant ion in the mid range is the y ion. Notice that only ammonia loss (-17) is noted for the y ions in Figure 2. The -17u loss is consistent for fragments containing the residues K, Q and N. Also note that the greatest ammonia loss is observed for fragment y9 which contains the greatest number of these residues. Figure 2.
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Shall we proceed onto the high mass range of the spectrum? Let's.
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| back | forward | |
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Reference Figuresb and y Ion Table
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Full Fragment Spectrum
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