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-De Novo Peptide Sequencing Tutorial Peptide Fragmentation Nomenclature b, y and a ions |
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click to see the full annotation |
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The most common peptide fragments observed in low energy collisions are a,
b and y ions, as described in the figure above. The b
ions appear to extend from the amino terminus, sometimes called the
N-terminus, and y ions appear to extend from the carboxyl terminus,
or C-terminus. While readily observed and diagnostic for b
ions, a ions occur at a lower frequency and abundance in relation to b
ions. The a ions are often used as a diagnostic for b ions, such that a-b
pairs are often observed in fragment spectra. The a-b pairs
are separated by 28u, the mass for the carbonyl, C=O. The fragment types listed above are the most common fragments observed with ion trap, triple quadrupole, and q-TOF mass spectrometers. Follow the link to see the fully annotated fragmentation nomenclature as proposed by Biemann. An important note: an earlier nomenclature was proposed by Roepstorff and Fohlman and later modified by Biemann. The Biemann adaptation has been widely accepted. Proceed onto the next page to learn a little more about b and y ions.
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