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N- and O-linked 
Protein Glycosylation


"N-Linked "


All N-linked carbohydrates are linked  through N-Acetylglucosamine and the amino acid asparagine as shown in  Figure 1.

The N-linked amino acid consensus sequence is Asn-any AA- Ser or Thr.  The middle amino acid can not be proline (Pro).  

Figure 1




"O-Linked "


Most O-linked carbohydrate covalent  attachments to proteins involve a  linkage between the  monosaccharide N- Acetylgalactosamine and the amino acids serine or threonine.

Currently there is not an O-linked amino acid consensus sequence.


Figure 2




IonSource Carbo Links

Monosaccharide table with structures

Simple monosaccharide mass table

Low mass CID marker ions

Carbohydrate mass spectrum pattern recognition table



References and External Links


IUPAC-IUB Joint Commission on Biochemical Nomenclature (JCBN), Nomenclature of glycoproteins, glycopeptides and peptidoglycans, Recommendations 1985.

NIH BUSM Mass Spectrometry Resource Supported by the NIH National Center for Research Resources for the development and application of mass spectral methods for biology and medicine with a primary emphasis on studies of oligosaccharides and glycoconjugates. Director Catherine Costello

O-GlycBase v5.00, World Wide Web Database Service, Center for Biological Sequence Analysis, Center for Biological Sequence Analysis, BioCentrum-DTU, Technical University of Denmark


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Last updated:  Tuesday, January 19, 2016 02:49:07 PM