IonSource Tutorial : "Introduction to Capillary Chromatography "


Capillary HPLC 
Abbreviations and Definitions


FSC (fsc)

Fused silica capillary.  Used as a pliable HPLC tubing for capillary HPLC, CE and GC. It is commonly used in LC/MS applications.


Sleeves are short lengths (1 to 2 cm) of PEEK™, teflon or other types of tubing that are placed over the ends of fsc to give physical stability when nuts and ferrules are swaged onto the fsc ends.


Polyetheretherketone.   It is commonly used as makeshift HPLC tubing. PEEK™ tubing is resistant to organic and inorganic solvents. The tubing is pliable and easily cut to length. It withstands presures to 400 bar and temperature to 100oC. PEEK™ is often used as FSC sleeve material.


Used to split HPLC flow. A tee junction where flow is brought in at a high rate and equally or differentially split between two exit ports.


Made of metal or polymeric material, unions are used to connect two pieces of tubing. A union can be used to connect tubing of equal or unequal diameter.
 Fingertights A one or two piece nut and ferrule that can be swaged by hand, usually made from PEEK™ or some other polymer.  Fittings made with fingertights have difficulty withstanding system pressures above 200 bar.


Trifluoroacetic acid. Organic modifier commonly used in HPLC solvents. Used to improve peak shape and recovery. Note: TFA has an ionization suppressive effect when used in conjunction with LC/MS and electrospray mass spectrometry.


The process of locking or crimping a ferrule onto HPLC tubing.

 Capillary column

Any HPLC column having an internal diameter equal to or less than 0.5 mm.

 Micro-bore column

Any HPLC column having an internal diameter equal to or greater than 0.5 mm and less than 2.1 mm.

  Narrow-bore column

 2.1mm ID diameter column





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Last updated:  Friday, April 29, 2005 08:17:36 AM